mes buffer pkames buffer pka

You want to make 1 L of a 1 M solution of MES (pH 6.2). We consider H2O(l) as 1 because it is not going to be relevant in finding our pH or pOH. And I don't know about The Henderson-Hasselbalch equation gives you a really quick The concentration, temperature, and ionic composition . 15 uses of MES Buffer you didn't know - Hopax Fine Chemicals It is a zwitterionic, morpholinic buffer that is useful for a pH range of 5.5 - 6.7 and commonly used for cell culture media, as a running buffer in electrophoresis, and for protein purification in chromatography. learn about titrations yet, that's fully fine. 1 0 obj is going to be positive. they're equal to each other or one is bigger than the other, we can immediately know This is, as written, it's the acid dissociation Good's buffers - Wikipedia our acid, so A minus. High concentrations of MES are toxic to most plants, but can be used in plant media at concentrations ~10 mM; (4) Electrophoresis and chromatography including capillary electrochromatography, gel filtration chromatography, phosphocellulose column chromatography, hydrophobic interaction chromatography, cation exchange chromatography and SDS-PAGE; Assume that the pH is 6 and the concentration is 100mM: (1) 0.1mol or 19.5g of MES was weighed and dissolved in 500mL of DEPC water; (2) The pH was adjusted to 6.0 with 1N or 10N NaOH solution; Application of Tris|CAS:77-86-1| in the field of coatings, Application of guanidine hydrochloride|CAS:50-01-1| in the treatment of cancer/tumor, Application of Tris|CAS:77-86-1| in eye drops, Application of Guanidine Hydrochloride|CAS:50-01-1| in Energy and Chemical Industry, Application of guanidine hydrochloride|CAS:50-01-1| in the field of graphene preparation, Guanidine HCL Guanidine hydrochloride CAS 50-01-1, 99.5% purity HEPES CAS:7365-45-9 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid, N-Cyclohexyl-3-aminopropanesulfonic acid CAS1135-40-6. Oh, sorry, if you haven't Direct link to s.m.guillaume's post at 1:32, correct me if I', Posted 7 years ago. 1. not super-intuitive sometimes. pH and pKa relationship for buffers (video) | Khan Academy Import fees:the customer must pay these fees directly to the delivery company (DHL) when receiving the product. Co., Ltd - Fine Chemicals Division. MES Free Acid is a buffer used often in plant tissue culture media with a pKA = 6.1 at 25C, 2-(N-morpholino)ethanesulfonic Acid MES, Free Acid JavaScript seems to be disabled in your browser. So what does this tell us? endobj pKa: 6.1. Generally, the pH value is set using NaOH/ . that the concentration of your acid and its 2/22/2021 8:55:39 AM. ratio is equal to one. to solve for this ratio that we might be interested in. MES is used as a buffering agent in biology and biochemistry. 5 ml of 10 M HCI 50 mL of 10 M HCI O 5 ml af 10 M NaOH 50 mL Show transcribed image text MES; Common buffer compounds used in biology; References This page was last edited on 7 February 2023, at 00:31 (UTC). TrisHCl (pKa = 8.06) and maleate (pKa = 6.26) have a working range of pH 5.08.6 and may be used successfully to buffer staining solutions (. MES Buffer | CAS 4432-31-9 - Products - Hopax Fine Chemicals We're saying that pH versus pK_a, this relationship, can tell us about A minus over HA, which is a ratio, but that in turn we can relate to just the relative size of HA versus A. Application: MES, Sodium Salt is a buffering agent used in biological and biochemical research including plant cell culture CAS Number: 71119-23-8 Purity: 99% Molecular Weight: 217.22 Molecular Formula: C 6 H 12 NO 4 SNa For Research Use Only. We can construct an acid dissociation expression for strong acids and calculate their Ka and pKa values. Useful pH Ranges of Selected BiologicalBuffers (25 C, 0.1 M) UsefulpKapKapKa pH67891011pH Range(at 20)(at 25)(at 37) MES5.5-6.7 6.16 6.10 5.97 BIS-TRIS5.8-7.2 - 6.50 6.36 ADA6.0-7.2 6.65 6.59 6.46 ACES6.1-7.5 6.88 6.78 6.54 PIPES6.1-7.5 6.80 6.76 6.66 MOPSO6.2-7.6 - 6.90 6.75 BIS-TRIS PROPANE6.3-9.5 - 6.8,9.0 - BES6.4-7.8 7.17 7.09 6.90 [3] MOPS buffer solutions become discolored (yellow) over time, but reportedly slight discoloration does not significantly affect the buffering characteristics.[4]. What would you add to this before you brought the volume to 1 L with water? Direct link to Ryan W's post The pKa is equal to the p. Tris buffers are used commonly in microtechnique applications involving molecular biological procedures. MES is not a good buffer for pH 4 because the pKa of MES is 6.1. is actually greater than the denominator, HA. [1] The buffer range is 5.5~6.7, pKa value of MES buffer is near to physiological pH, it is used in the following experiments: (1) Replace highly toxic cacodylate, and ion buffer citrate and malate; (2) Buffered media for bacterial, yeast and mammalian cells. I want to produce a 0.1 M MES buffer solution at different pH values to check for enzyme activity. The melting point is approx. ;|c]?^~OorOO7/}_}w{/8|f>k{?`8tmz}v=]>F/@-~OyH?h`/0cW`{7r\Nq}8v/?Gy;}cOW.7=~4\\Gh0N =oOaw7{_u~dx{gq9ebl,\c~|wh{~;a?P{gru3]0}28|l~M@w~%vr.D?c_{t&4e< #P;MGpTo@Hkc?}2r6`vB_?^yhq/q,{px76? Plant Microtechnique and Microscopy, CACODYLATE BUFFER; PH 5.07.4, PKA = 6.27. Just ignore what I just said (laughs), but if you have, the moral is just that, this is a really, really For example, a buffer with a pH of 6.8 has a pH buffering range of 5.8-7.8. Keep buffer concentration as low as possible yet enough to maintain pH. And as you can see up here, an acid and its conjugate base are just related by the fact Add the following amounts of 0.2 M HCl per 100 ml cacodylate stock solution, followed by the addition of DI to a final volume of 400 ml, to obtain 0.05 M cacodylate buffer at the desired pH (Dawes, 1971). please read on, stay posted, subscribe, and we welcome you to tell us what you think. And it just has a special Direct link to Jesi Buckley's post At 5:46, she mentions the, Posted 3 years ago. It has pKa value of 6.15 at 20C. Any time you have a buffer and the pH of your solution is equal to the pK_a of your buffer, you immediately know Direct link to Jesi Buckley's post At 3:55, shouldn't the pH, Posted 4 years ago. x}"tOUFC],yyTLk{'reV&@R\ ra!7_?>9472Mwt_vxga8g=r.X{?? K_a is just equal to H_three O plus times A minus all over the concentration of HA, and we don't include water [ 5] Other Notes 2 0 obj MES (buffer) - Wikipedia Direct link to dilu.ruwangika's post what happens to the buffe, Posted 4 years ago. InChI=1S/C6H13NO4S/c8-12(9,10)6-3-7-1-4-11-5-2-7/h1-6H2,(H,8,9,10), InChI=1/C6H13NO4S/c8-12(9,10)6-3-7-1-4-11-5-2-7/h1-6H2,(H,8,9,10), Except where otherwise noted, data are given for materials in their, https://en.wikipedia.org/w/index.php?title=MES_(buffer)&oldid=1141241576, Chemical articles with multiple compound IDs, Multiple chemicals in an infobox that need indexing, Chemical articles with multiple CAS registry numbers, Articles with changed ChemSpider identifier, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License 3.0, This page was last edited on 24 February 2023, at 02:15. Absorbance (1.0 M, H2O, 260 nm):0.05. When pH is equal to pK_a, we're raising 10 to the zeroth power. In water, HA can dissociate into A- and H+. Assay (titr. So the negative log of 5.6 times 10 to the negative 10. Language links are at the top of the page across from the title. We did it in just a few minutes, so it's okay if you don't It is recommended for separating small- to medium-sized proteins. All rights reserved. And then that means you So anything to the zeroth power is equal to one. PDF Biological Buffers - ITW Reagents It is not absorbed through cell membranes, and it is essentially transparent to UV light. MES monohydrate Buffer - Hampton Research For a buffer to be most effective . - [Voiceover] We're gonna Synonyms: MES or 2-(N-morpholino)ethanesulfonic acid or 4-morpholineethanesulfonic acid monohydrate C 6 H 13 NO 4 S H 2 O M r 213.25 CAS Number [145224-94-8] EC Number 224-632-3 Beilstein Registry Number 329824236 RTECS QE3479500 MDL Number MFCD00149409 Purity 99.5% pKa 6.1 at 25C. MES is the common name for the compound 2-(N-morpholino)ethanesulfonic acid. let's do a quick review of what exactly is a buffer. The choice of buffer is not only according to the required buffer range and capacity of buffer solution, but also according to the specific experimental content. HR2-943-07 - MES monohydrate pH 5.8 - 1.0 M solution is a Custom Shop reagent and is used to reproduce and optimize Crystallization Reagents for use with the Silver Bullets kit. and easy way of doing that. of A minus and HA. It has minimal UV absorption and is often used in plant cell cultures and as a running buffer for bis-tris gels. deprotonated). ,d6R J%:LN*0B1'@PP">& "7/ubjM~O~O~OXTGl5-}YL+hdyd24a. So there are two other possibilities for pH and pK_a. HEPES Sodium is considered to be non-toxic to culture cell . When pH is equal to pK_a, we're raising 10 to the zeroth power. Terms & Conditions, By submitting your email address, you are agreeing to our, Murashige & Skoog Medium with MES Buffer & Vitamins, MES-buffered saline (5X, pH 7.4), low endotoxin, ROCHE DOTAP Liposomal Transfection Reagent, N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N, ROCHE DOTAP Liposomal Transfection Reagent, N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-trimethylammonium methyl-sulfate. (5) Complex with organotin (IV) molecules as antitumor agents. Used for mammalian cell culture 8. MOPS is a similar pH buffering compound which contains a propanesulfonic moiety instead of an ethanesulfonic one. NuPAGE MES SDS Running Buffer (20X) is formulated for running proteins on NuPAGE Bis-Tris gels. [2] MES is highly soluble in water. Mix appropriate volumes of stock and add an equal volume of distilled water to make a final 0.1 M Srensens phosphate buffer solution (Srensen, 1909; Gomori, 1955). Aqueous dispersion (liposomes) in MBS (MES-buffered saline, pH 6.2) (bottled under argon), 1mg/ml, sterile-filtered. PDF FT-062000 Good's buffers (biological buffers) - interchim.fr MES Buffer is a remarkably chemically and enzymatically stable zwitterionic buffer. Solutions are stable at 2-8C for months. Calculate the composition of buffer solution containing CH3COOH. you, but I actually find, well, (laughs) I find logs And it's saying that these MES, Free Acid - PhytoTech Lab The last scenario, pH is less than pK_a, well in that case, we're raising 10 to a negative number, because we're subtracting a bigger 4 0 obj In short, the stronger the acid, the smaller the pKa value and strong acids have weak conjugate bases. Also used in c MES-Buffer has a pKa value near physiological pH, it is not absorbed through cell membranes, and it is essentially transparent to UV light. ;jNWEz-}/Wn]})asgR.1"B0q"@l:F 16MUed98:`c#-1M0Reru! The melting point is approx. Mes-Buffer at Thomas Scientific MES does not form radical species, making it suitable for redox studies 5. Autoclaving is not recommended for any sulfonic acid buffer. Further reading: Useful pH range of Biological Buffers. Mix the three purified enzyme components of NBDO in 50 mM MES buffer at pH 6.8 containing 100 M (NH 4) 2 Fe(SO 4) 2 to final concentrations of 0.3 M reductase, 3.6 M ferredoxin, and 0.3 M oxygenase.. 2. Prepare a 0.2 M stock solution of sodium cacodylate in water (4.28 g/100 ml). Expert Answer. Direct link to Tzviofen 's post Why are all these eqautio, Posted a year ago. Compound name: MES buffer. acid, you wanna add some base. &FubS-MQdNVH5e5 These values indicate that the products of a strong acid reaction are heavily favored compared to the reactants. NuPAGE MES SDS Running Buffer (20X) - Thermo Fisher Scientific Lah, MS; Palfey, BA; Schreuder, HA; Ludwig, ML. HEPES is a similar pH buffering compound that contains a piperazine ring. A buffer consists of a weak acid (proton donor, HA) and its conjugate base (proton acceptor, A -). MES Buffer 0.1M pH 4.7 - Buffers and Reagents We're going to start with the simplest possible scenario, which is that pH is equal to pK_a. Further reading: How to choosing the biological buffer you need: By ph and pKa. ^O55% (AmAdXG`VdAM;l9@j{? Lle}?- mUqHIegj9M8QSN+lA=&UKK [@lv9^:cGggbO&a)vU=O'L+& .sp:RtZV_ l_'_'~{LI$/ZO;Z fsAqpQc5Q)u668rLJoiNit$[0OCaBapcIV5-;FEYj7($oJFeDz$>64XjL7.Qih mFu~V#R3tsd}sQIH\ So if you know the pH and you know it's bigger Mes 145224-94-8 A lot of times, you just wanna know, you know, what's in your solution, depending on what you MES was developed as one of 'Good' stamps in the 1960s. Text . ~k {,j3,vY"ZX kH|{/rOr8 sX+G(ux~z}7mH;#G{K=5W?77|wkk8\dn^54n?7y6q7?S/O>`}@Lgwvfy~_Q8K|7IRn_Yn,lo!7|w-t_P[?9Y|n^xB.WFG^;=^is2]%7Nzou uI#vl+cq};HslxG#qS8 eJyQ}EpVd S;Zufhx4Fq?legnL{bEkLC0ZKZY/#y!_MY\)k{?lW10SA:tW_9c_\z]kj/M7#5[@vRwwe}F'Ke\XnqW}C6~uw%w\ KTpQdpO7cug?WOvn_[.lUWRcTXQdokka15ot_a.8o][2Q^Iyr'5~@0J,vow c\/}R}e(F8,oSS\f.?BNdp.]cq'}LBbo!dbo8l=V/B;EvU5K{|Ruk0Mosl{_js6~x}6v;0c9n=I.;Z_?+0h_:GZ")o,{`\pq] MES, Free Acid, Ultrol Grade - CAS 4432-31-9 - Calbiochem A In addition, the experimenter should pay attention to the sterilization of containers, water and other additives. HEPES - aladdin-e.com Direct link to Ramisha Ghazi's post Calculate the composition, Posted 2 years ago. It has p Ka value of 6.15 at 20 C. <> Crystal structures of mutant Pseudomonas aeruginosa p-hydroxybenzoate hydroxylases: the Tyr201Phe, Tyr385Phe, and Asn300Asp variants. Hopax is a manufacturer and supplier of MES (CAS 4432-31-9), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research. Not Intended for Diagnostic or Therapeutic Use. Is going to give us a pKa value of 9.25 when we round. Suitable for most toxicity studies 9. Impurities:Endotoxin 50 EU/gYeast and Mold 100 CFU/gBioburden 100 CFU/gAl 5.0 ppmAs 3.0 ppmBa 5.0 ppmBi 5.0 ppmCa 20.0 ppmCd 5.0 ppmCo 5.0 ppmCr 5.0 ppmCu 5.0 ppmFe 5.0 ppmLi 5.0 ppmMg 5.0 ppmMn 5.0 ppmMo 5.0 ppmNa 20.0 ppmNi 5.0 ppmPb 5.0 ppmSb 5.0 ppmZn 5.0 ppm. The other thing that you can use the Henderson-Hasselbalch equation to tell you is the relationship between A minus and HA which is something you might wanna know. You weigh out 1 mole of the basic (unprotonated) form of MES and dissolve it in 750 ml water. }}h7~z1 :MK 1)Select all the products and quantities you would like to order and click on Add to cart. Direct link to Khan academy's post No, as the 10 is added du, Posted 6 years ago. MOPS - Wikipedia Not for use in diagnostic procedures. If the product you are interestedis available only in bulk quantities, click on "Request a Quotation". We can have a pH that's greater than pK_a for your buffer, and you can have a pH that is less than you It also emits toxic fumes upon combustion, including carbon monoxide, nitrogen oxide and sulfur oxides. To make a 1 L solution of 0.5M MES, use the table below to estimate the required volume of base for a given pH: Starting pH: 3.23 Adjust pH with: 10N NaOH And specifically, we're p Ka: Because most biological reactions take place near-neutral pH between 6 and 8, ideal buffers would have pKa values in this region to provide maximum buffering capacity there. Presented here are three common formulations (Mishkind, Sodium cacodylate buffer [Na(CH3)2 AsO2 3H2O] is a alternative to Srensens phosphate buffer. We are sad to see you go. MES, Sodium Salt | CAS 71119-23-8 | SCBT - Santa Cruz Biotechnology Which tells us that this % So if we are throwing them into water, the Hydrogen Ions begin to mix making more H2O(l) molecules. MES has a lower pKa than MOPS, making the MES SDS Running Buffer faster than the MOPS SDS running buffers. It's very easy to derive. Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA). Why are all these eqaution dealing with weak acids, why doesn't it work for strong acids?! Applications and Preparation of MOPS and MES Buffer - Yacooscience ta 2aur;V.H'LuT?b>x*:7"Y%sujq.oKLD s sJs+H+HpPA7t'jaVrO7$^m|g6q48Ow*1Y\x Use our interactive chart below to compare the useful pH range of this product with other buffers. Crystallization Plates, Hardware & Accessories, Crystallization grade MES monohydrate for formulating screens or for optimization, Formulated in Type 1+ ultrapure water: 18.2 megaohm-cm resistivity at 25C, < 5 ppb Total Organic Carbon, bacteria free (<1 Bacteria (CFU/ml)), pyrogen free (<0.03 Endotoxin (EU/ml)), RNase-free (< 0.01 ng/mL) and DNase-free (< 4 pg/L). With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH. MES 1-hydrate for buffer solutions MES 1-hydrate for molecular biology MES 2-(N-Morpholino)-ethanesulfonic acid 6.10 5.5 - 6.7 + -0.011 . Also known as [2-(N-Morpholino) ethanesulfonic Acid]. Identify which Good's buffers are most effective near pH 7.0 and near pH 6.0. The pKa is equal to the pH in that scenario, Buffers, titrations, and solubility equilibria. How to prepare the buffer solution? Hopax is a manufacturer and supplier of MES (CAS 4432-31-9), a zwitterionic biological buffer often used as a buffering agent in biological and biochemical research. going to be talking mostly about this in terms of the SODIUM ACETATE; PH 3.6-5.6, PKA = 4.76 on reaction for HA and since it's an equilibrium we can write an expression called K_a, which is just the equilibrium Direct link to Amanda Saideepane's post If exactly half the amoun, Posted 2 years ago. Good's buffers are compounds that are useful near neutral pH. Buffered saline solutions are used frequently when performing immunolocalization experiments. Direct link to Ernest Zinck's post Chemical equation: B + H, Posted 7 years ago. High concentrations of MES are toxic to most plants, but can be used in plant media at . GLYCINE-HCL; PH 2.2-3.6, PKA = 2.35 Combine 25 ml 0.2 M glycine and x ml HCl and dilute to 100 ml with DI (Dawson, et al., 1969). MES buffer - Microbiologie clinique ): 99% |CAS No:4432-31-9|Useful pH range:5.5 6.7 MES is a zwitterionic buffer. probably know the pK_a and you know the concentrations In personal care and cosmetics applications, MES can protect the active ingredients of the formulations against degradation. power is equal to one. Titrate HR2-587 to the desired pH using NaOHHR2-587 Measured pH Range: 3.2 - 3.4 at 25CHR2-587 Measured Conductivity Range: 166.4 - 2440 S/cm at 25CHR2-587 Measured Refractive Index Range: 1.34655 - 1.34731 at 20C, HR2-787 has been titrated to pH 6.5 using NaOHHR2-787 Measured Conductivity Range: 19.2 - 25.6 mS/cm at 25CHR2-787 Measured Refractive Index Range: 1.36113 - 1.36251 at 20C. just when you're talking about buffers by themselves, but also when you're doing titrations. > It has been tested and recommended for polyacrylamide gel electrophoresis. pKa 25C (1) 3.1 (2) 4.8 (3) 6.4 Sodium cacodylate trihydrate pKa 25C 6.3 MES monohydrate pKa 25C 6.1 ACES pKa 25C 6.8 PIPES pKa 25C 6.8 Imidazole pKa 25C 7.0 MOPSO pKa 25C 6.9 HEPES sodium pKa 25C 7.5 HEPES pKa 25C 7.5 BIS-TRIS propane pKa 25C (1) 6.8 (2) 9.0 BES Cacodylate was introduced for electron microscopy applications by Sabatini. contains both a weak acid, which generically we write as HA, and it also contains in equilibrium the conjugate base of Components: MES Monohydrate(500 mM), Sodium Hydroxide(for pH adjustment), Water(Rest) Method: Prepared in 18.2 megohms-cm 1 water and filtered through 0.22-micron filter. And this comes up a lot not This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory. 5)You will see a message confirming the order. Language links are at the top of the page across from the title. (3) Make the volume to 1L, preserve at 4C. two things are related to the relative size of pH and pK_a. Specifically, sodium acetate buffer (pKa 4.75) ranging from pH 3.75-5.75, MES buffer (pKa 6.1) from pH 5.1-7.1, PIPES buffer (pKa 6.75) from 5.75-7.75 and HEPES buffer (pKa 7.5) from 6.50 . at all the possible scenarios for these three things. simplest possible scenario, which is that pH is equal to pK_a. (7) For study on electron transport and phosphorylation of chloroplast sample preparation. (,qT)NmNA`ZSDtVi Buffers work better within 1 unit of the pKa. Solved Good's buffers are compounds that are useful near - Chegg It is a structural analog to MES, [1] and like MES, its structure contains a morpholine ring. So a buffer is something that contains an aqueous solution. 4)Write down the buyer and shipping information. [1] MES is also useful as a non-coordinating buffer in chemistry involving metal ions, as many common buffers (e.g. to get rid of the log by raising both sides to the 10th power. because it's a pure liquid, so we assume that the Contact with this buffer is hazardous;[5] skin or eye exposure should be cleaned well with water and medical aid should be sought in the case of eye exposure, swallowing or inhalation of dust. How to choose the right biological buffer - Hopax Fine Chemicals remember this all the time. From Plant & Animal Microtechnique, SE Ruzin, Oxford Univ Press, 1999, From Ruzin, 1999. Which acid is used to adjust MES buffer? | ResearchGate Its chemical structure contains a morpholine ring, MES buffer has a buffer range of pH 5.8 to 6.5. Which acid is used to adjust MES buffer? In the aqueous buffer solution, H3O+, HA and H+ exist in equilibrium with each other. The temperature dependence of a buffer system is expressed as d(pKa)/dT, which describes the change of the pK a at an increase of temperature by 1C. So we're gonna plug that into our Henderson-Hasselbalch equation right here. 3 0 obj Biochemistry 33, 1555- 1564, 1994. Optimize Reagents Not all buffers will be used. 3FV4ZrPmLRhbiM}~^RiJc0;SMa PDF Buffer Table - Hampton Research So just to wrap up, we can look at the Henderson-Hasselbalch equation and we can just look at the relationship between pK_a and pH, and depending on whether Thermo Fisher Scientific. MES . Standard mixing tables using stock solutions to prepare a buffer of a given pH have been published. Why is this important for biologists? These buffers were developed with the following criteria in mind: midrange pKa, maximum water solubility and minimum solubility in all other solvents, minimal salt effects, minimal change in pKa with temperature, chemically and enzymatically stable, minimal absorption in visible or UV spectral range and reasonably easily synthesized. [2] Usage above 20 mM in mammalian cell culture work is not recommended. other videos introducing it and also deriving it, than the pK_a of your buffer, buffer's acid, to be more specific, then you immediately know that you have more conjugate base around than your acid. Mes at Thomas Scientific So I'm actually going So pKa is equal to 9.25. thing to remember. When MES solutions are stored at room temperature, they can turn yellow within six months. MES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al.